e2 640 100 recombinant e1 Search Results


anti human cd27 rea499 155gd  (Miltenyi Biotec)
94
Miltenyi Biotec anti human cd27 rea499 155gd

Anti Human Cd27 Rea499 155gd, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2+640+100+recombinant+e1/CD27+Antibody%2C+anti-human%2Fmouse%2C+REAfinity/pmc09243624-11-0-8
Average 94 stars, based on 1 article reviews
anti human cd27 rea499 155gd - by Bioz Stars, 2026-09
94/100 stars
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his ubch7  (R&D Systems)
94
R&D Systems his ubch7

His Ubch7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2+640+100+recombinant+e1/Recombinant+Human%2FMouse%2FRat+UbcH7%2FUBE2L3+Protein%2C+CF/pm37863914-318-5-13
Average 94 stars, based on 1 article reviews
his ubch7 - by Bioz Stars, 2026-09
94/100 stars
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ube2l3  (R&D Systems)
93
R&D Systems ube2l3
Figure 3. UBR5 ubiquitylates transcriptional regulators (A) Endogenous UBR5 was purified from HeLa cells and incubated with E1, UBE2D3, ubiquitin, and 35S-labeled targets produced by in vitro transcription/ translation (IVT/T). Substrate modification was analyzed by autoradiography (left panel: a silver-stained gel of UBR5). (B) 35S-labeled MCRS1 was incubated with UBR5, E1, <t>UBE2L3,</t> and ubiquitin. (C) UBR5, UBR5C2768S, and UBR5DUBA were incubated with 35S-labeled MCRS1. (D) 35S-labeled MCRS1 was incubated with UBR5, E1, UBE2D3, and ubiquitin mutants and analyzed for ubiquitylation as above. (E) 35S-labeled MCRS1-Ub4 was incubated with UBR5, E1, UBE2D3, and ubiquitin mutants. See also Figure S3.
Ube2l3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2+640+100+recombinant+e1/Recombinant+Human%2FMouse%2FRat+UbcH7%2FUBE2L3+Protein%2C+CF/pm37478862-281-16-17
Average 93 stars, based on 1 article reviews
ube2l3 - by Bioz Stars, 2026-09
93/100 stars
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mouse tlr11 alexa fluor« 647 conjugated antibody  (R&D Systems)
N/A
The Mouse TLR11 Alexa Fluor« 647 conjugated Antibody from R D Systems is a rat monoclonal antibody to TLR11 This antibody reacts with mouse The Mouse TLR11 Alexa Fluor« 647 conjugated Antibody has been validated
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s100a2 antibody (biotin)  (biorbyt)
N/A
Rabbit polyclonal antibody against S100A2 conjugated to Biotin. Isotype Note: IgG Host Note: Rabbit Conjugation Note: Biotin Reactivity Note: Human Application Note: ELISA
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recombinant kif3a antibody  (Aladdin Scientific Corporation)
N/A
Rabbit anti Human KIF3A Antibody, Recombinant, could be used for WB and so on.Application:WB: 1/500-1/1000Protein FunctionMicrotubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.
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human nov ccn3 antibody  (R&D Systems)
N/A
The Human NOV CCN3 Antibody from R D Systems is a mouse monoclonal antibody to NOV CCN3 This antibody reacts with human The Human NOV CCN3 Antibody has been validated for the following applications Western
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alpha adaptin antibody (8r8b6)  (Novus Biologicals)
N/A
The alpha Adaptin Antibody (8R8B6) from Novus is a alpha Adaptin antibody to alpha Adaptin. This antibody reacts with Human, Mouse, Rat. The alpha Adaptin antibody has been validated for the following applications: Western Blot,
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bovine coronavirus bcov spike protein (recombinant 10his, n-terminus) (aa326-540)  (Lifespan Biosciences)
N/A
BCoV Spike Protein LS-G64076 is a Recombinant Bovine Coronavirus BCoV Spike aa 326-540 produced in E. coli with 10His, N-terminus tag(s).
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calciseptine (recombinant)  (Aladdin Scientific Corporation)
N/A
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
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mouse tlr11 alexa fluor« 700 conjugated antibody  (R&D Systems)
N/A
The Mouse TLR11 Alexa Fluor« 700 conjugated Antibody from R D Systems is a rat monoclonal antibody to TLR11 This antibody reacts with mouse The Mouse TLR11 Alexa Fluor« 700 conjugated Antibody has been validated
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mouse tlr11 alexa fluor« 594 conjugated antibody  (R&D Systems)
N/A
The Mouse TLR11 Alexa Fluor« 594 conjugated Antibody from R D Systems is a rat monoclonal antibody to TLR11 This antibody reacts with mouse The Mouse TLR11 Alexa Fluor« 594 conjugated Antibody has been validated
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Image Search Results


Journal: Cell Reports Methods

Article Title: Development of an HIV reporter virus that identifies latently infected CD4 + T cells

doi: 10.1016/j.crmeth.2022.100238

Figure Lengend Snippet:

Article Snippet: Anti-human CD27 (REA499) 155Gd, coupled in house , Miltenyi Biotec , Cat#: 130122295, RRID: AB_2801876.

Techniques: Virus, Plasmid Preparation, Recombinant, Cell Isolation, Staining, Software

Figure 3. UBR5 ubiquitylates transcriptional regulators (A) Endogenous UBR5 was purified from HeLa cells and incubated with E1, UBE2D3, ubiquitin, and 35S-labeled targets produced by in vitro transcription/ translation (IVT/T). Substrate modification was analyzed by autoradiography (left panel: a silver-stained gel of UBR5). (B) 35S-labeled MCRS1 was incubated with UBR5, E1, UBE2L3, and ubiquitin. (C) UBR5, UBR5C2768S, and UBR5DUBA were incubated with 35S-labeled MCRS1. (D) 35S-labeled MCRS1 was incubated with UBR5, E1, UBE2D3, and ubiquitin mutants and analyzed for ubiquitylation as above. (E) 35S-labeled MCRS1-Ub4 was incubated with UBR5, E1, UBE2D3, and ubiquitin mutants. See also Figure S3.

Journal: Cell

Article Title: Orphan quality control shapes network dynamics and gene expression.

doi: 10.1016/j.cell.2023.06.015

Figure Lengend Snippet: Figure 3. UBR5 ubiquitylates transcriptional regulators (A) Endogenous UBR5 was purified from HeLa cells and incubated with E1, UBE2D3, ubiquitin, and 35S-labeled targets produced by in vitro transcription/ translation (IVT/T). Substrate modification was analyzed by autoradiography (left panel: a silver-stained gel of UBR5). (B) 35S-labeled MCRS1 was incubated with UBR5, E1, UBE2L3, and ubiquitin. (C) UBR5, UBR5C2768S, and UBR5DUBA were incubated with 35S-labeled MCRS1. (D) 35S-labeled MCRS1 was incubated with UBR5, E1, UBE2D3, and ubiquitin mutants and analyzed for ubiquitylation as above. (E) 35S-labeled MCRS1-Ub4 was incubated with UBR5, E1, UBE2D3, and ubiquitin mutants. See also Figure S3.

Article Snippet: E1was purified as described inMeyer and Rape (2014)96 while commercially available UBE2D3 (R&D Systems, E2-627-100) and UBE2L3 (R&D Systems, E2-640-100) were used.

Techniques: Incubation, Ubiquitin Proteomics, Labeling, Produced, In Vitro, Autoradiography, Staining

Figure 6. UBR5 provides orphan quality control (A) UBR5 was incubated with TAMRA-labeled MCRS1 degron, E1, UBE2L3, and ubiquitin. Ubiquitylation was monitored by fluorescence. (B) UBR5 ubiquitylated TAMRA-labeled SPT5 degron peptides. (C) UBR5, UBR5C2768S, and UBR5DUBA were incubated with the SPT5 degron, E1, UBE2L3, and ubiquitin. (D) BACH2 protects MAFF from UBR5-dependent degradation. The MAFF reporter was expressed in cells depleted of UBR5 and/or co-expressing BACH2, as indicated. MAFF stability was determined by flow cytometry. (E) The MCRS1 reporter is stabilized by CCDC85B. (F) The RUVBL2 reporter is stabilized by RUVBL1. (G) The OCT4 reporter is stabilized by SOX2. (H) BACH2 protects endogenous MAFF from UBR5-dependent degradation. 293T cells were depleted of BACH2 and/or UBR5, and endogenous MAFF was visualized by western blot analysis. (I) SOX2 protects endogenous OCT4 from UBR5-dependent degradation in H1 hESCs.

Journal: Cell

Article Title: Orphan quality control shapes network dynamics and gene expression.

doi: 10.1016/j.cell.2023.06.015

Figure Lengend Snippet: Figure 6. UBR5 provides orphan quality control (A) UBR5 was incubated with TAMRA-labeled MCRS1 degron, E1, UBE2L3, and ubiquitin. Ubiquitylation was monitored by fluorescence. (B) UBR5 ubiquitylated TAMRA-labeled SPT5 degron peptides. (C) UBR5, UBR5C2768S, and UBR5DUBA were incubated with the SPT5 degron, E1, UBE2L3, and ubiquitin. (D) BACH2 protects MAFF from UBR5-dependent degradation. The MAFF reporter was expressed in cells depleted of UBR5 and/or co-expressing BACH2, as indicated. MAFF stability was determined by flow cytometry. (E) The MCRS1 reporter is stabilized by CCDC85B. (F) The RUVBL2 reporter is stabilized by RUVBL1. (G) The OCT4 reporter is stabilized by SOX2. (H) BACH2 protects endogenous MAFF from UBR5-dependent degradation. 293T cells were depleted of BACH2 and/or UBR5, and endogenous MAFF was visualized by western blot analysis. (I) SOX2 protects endogenous OCT4 from UBR5-dependent degradation in H1 hESCs.

Article Snippet: E1was purified as described inMeyer and Rape (2014)96 while commercially available UBE2D3 (R&D Systems, E2-627-100) and UBE2L3 (R&D Systems, E2-640-100) were used.

Techniques: Control, Incubation, Labeling, Ubiquitin Proteomics, Expressing, Cytometry, Western Blot